Samantha G Zeitlin | UNIVERSITY OF CALIFORNIA SAN DIEGO

RCID: 643625
Link to this profile: http://www.researchcrossroads.org/Researchers/643625

Samantha G Zeitlin

UNIVERSITY OF CALIFORNIA SAN DIEGO


Department Department Of Cellular And Molecular Medicine Website Degrees PhD Interests DNA repair, chromatin, mitosis, cytokinesis, stem cells, epigenetics, transposable elements, repetitive sequences, nucleotide metabolism, nucleoli All Funding $ 138,832

Biography

Dr. Zeitlin attended the Thomas Jefferson High School for Science and Technology in Alexandria, VA, where she did her external senior research project with Ed Gelmann's lab in the Department of Urology at the Lombardi Cancer Center of Georgetown University. She attended the University of Pennsylvania in Philadelphia and majored in Biochemistry, where she ran the departmental seminar series and received the Helix Award. As her main extracurricular activity, she worked with David Christianson in the Department of Chemistry, where she solved crystal structures, and with Haig Kazazian in the Department of Genetics, where she studied retrotransposition in cultured living human cells. For her PhD, she traveled to The Scripps Research Institute in San Diego, CA. She did two six-month rotations, one with Roger Beachy (now at the Danforth Center in St. Louis) studying the struture of mutant tobacco mosaic virus using electron microscopy
and one with Alan McLachlan (now at the University of Chicago) studying hepatitis virus B (HBV) replication in vitro. For her thesis work, she joined Kevin Sullivan's lab (now at Galway University in Ireland) and characterized the phosphorylation of centromere protein A (CENP-A) by the kinase now known as Aurora B. She found that a mutant of CENP-A that is not a substrate for Aurora B causes a delay in the final stage of cytokinesis, which surprisingly linked an event in early G2 with the end of cell division hours later. For her postdoctoral training, she joined John Newport's lab at UCSD, where she found that DNA repair is required for CENP-A assembly onto DNA in vitro in Xenopus egg extracts. She then joined Jean Wang's lab to test whether this was also true in human cells in culture, and found that it is. More recently she joined Don Cleveland's lab and has been working with human embryonic stem cell lines in collaboration with Larry Goldstein's lab. She has also used a laser, in collaboration with Michael Bern's lab, to demonstrate that DNA damage is sufficient to recruit CENP-A. She is currently seeking a tenure-track assistant professor faculty position where she hopes to continue her independent research into the connection between CENP-A, centromeres, and DNA repair.

Grants (Principal Investigator)

Grants for which Samantha G Zeitlin acted as the principal investigator

Title/Granting Organization		Fiscal Years	Total Funding
Biochemical Basis Of Cenp-a Epigenetic Inheritance
	National Institute of General Medical Sciences(NIGMS)	2003 - 2005	$ 138,832

Publications

(% contribution)
1. Zeitlin SG* (75%), Chapados BR, Slupphaug G, Cleveland DW and Wang JY.
Centromere assembly mediated by a DNA repair enzyme
(submitted to Current Biology). *corresponding author
2. Zeitlin SG* (80%), Baker NM, Wang JY, Berns MW and Cleveland DW.
DNA damage is sufficient to recruit Centromere protein A (to be submitted to Nature).
*corresponding author
3. Orjalo A, Arnaoutov A, Boyarchuk Y, Zeitlin SG (15%), Shen Z, Briggs S, Dasso M
and Forbes DJ. (2006) The Nup107-160 nucleoporin complex is required for correct
spindle assembly at mitosis. Molecular Biology of the Cell 17:3806-18.
4. Alvarez D, Chou CJ, Latella L, Zeitlin SG (15%), Ku S, Puri PL, Dervan PB, and
Gottesfeld JM. (2006) A two-hit mechanism for pre-mitotic arrest of cancer cell
proliferation by a polyamide-alkylator conjugate. Cell Cycle 5:1537-48.
5. Zeitlin SG* (75%), Patel S, Kavli B and Slupphaug G. (2005) Xenopus CENP-A
assembly into chromatin requires base excision repair proteins. DNA Repair. 4:
760-772. *corresponding author
6. Schröfelbauer B, Yu Q, Zeitlin SG (15%) and Landau NR. (2005) HIV-1 accessory
protein Vpr protects the viral genome by inducing the degradation of the uracil DNA
glycosylases UNG2 and SMUG-1. Journal of Virology 79: 10978-87.
7. Zeitlin SG (85%), Shelby RD, and Sullivan KF. (2001) CENP-A is phosphorylated by
Aurora B kinase and plays an unexpected role in completion of cytokinesis. Journal
of Cell Biology. 155:1147-57.
8. Zeitlin SG (50%) and Sullivan KF. (2001) Animal cytokinesis: Breaking up is hard to
do. Current Biology 11:R514-6.
9. Zeitlin SG (50%), Monier K, Sullivan KF. (2001) Phospho-histone
immunofluorescence for staging of G2 and prophase (Methods Review). Chemtracts
Biochemistry and Molecular Biology 14: 557-562.
10. Zeitlin SG (50%), Barber C, Allis CD, Sullivan KF. (2001) Differential regulation of
CENP-A and H3 phosphorylation in G2/M. Journal of Cell Science 114: 653-661.
11. Boriack-Sjodin PA, Zeitlin S (35%), Chien HH, Crenshaw L, Gross S, Dantanarayana
A, Delgado P, May JA, Dean T, Christianson DW. (1998) X-ray crystallographic
studies of Thienothiazine-6-sulfonamide binding to carbonic anhydrase II.
Protein Science 7: 2483-2489.
12. Berchem G, Bosseler M, Sugars L, Voeller J, Zeitlin S (25%), Gelmann EP. (1995)
Androgens induce resistance to bcl-2 mediated apoptosis in LNCaP prostate cancer
cells. Cancer Research 55: 735-738.

Patents

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